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This selection cassette and a lox P site was inserted 200 bp downstream of exon 4 of the targeted gene, and another lox P site was inserted 700 bp upstream of exon 3.

This construct was electroporated into 129 derived R1 embryonic stem (ES) cells.

When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exons 3 and 4 deleted in the cre-expressing tissue(s).

The Donating Investigator reports that the presence of the FRT site flanked PGK-Neo cassette has no detectable effect on gene expression in this strain.

Officers said most of the men arrested reside in Gainesville and Alachua County.

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These floxed mutant mice possess lox P sites flanking exons 3 and 4 of the Chat gene.

This strain may be useful for generating conditional mutations for studying cholinergic neurotransmission and non-neuronal cholinergic systems.